| 621 | 9 | 82 |
| 下载次数 | 被引频次 | 阅读次数 |
针对酸性矿山废水高浓度硫酸盐和金属离子以及低pH的污染情况，从矿区煤矸石堆的底泥和浸出液中筛选鉴定出一株硫酸盐还原菌，根据其形态特征以及16SrRNA基因序列进行鉴定，研究碳源、温度、pH、初始硫酸盐浓度对该菌株生长和还原硫酸盐的影响，并利用该菌株处理酸性矿山废水。菌株经过16SrRNA基因测序后，鉴定为Citrobacter freundii,命名为S1(NCBI登录号：PP273014)。菌株S1在35℃、pH为7.0、SO42-浓度2 000 mg/L的条件下生长最旺盛。菌株S1对酸性矿山废水中TFe、Mn~2+、Zn~2+和SO42-均有一定的处理效果，接种7 d后去除率分别为81.96%、35.08%、52.92%和63.24%,废水pH从4.38提高到了6.6左右。
Abstract：Address to the pollution of acid mine wastewater with high concentration of sulfate and metal ions and low pH value, a strain of sulfate-reducing bacteria was isolated from the bottom sediment and leachate of the coal gangue pile in the mining area.This strain was identified according to its morphological characteristics and 16SrRNA gene sequence.The effects of carbon source, temperature, pH value and initial sulfate concentration on the growth and sulfate reduction of the strain were studied, and the strain was used to treat acid mine wastewater.The strain is identified as Citrobacter freundii after 16SrRNA gene sequencing and named S1(NCBI accession number: PP273014).Strain S1 exhibits the greatest growth rate at 35 ℃,pH value of 7.0,and sulfate concentration of 2 000 mg/L.Strain S1 demonstrates a certain efficacy in the treatment of TFe, Mn~2+,Zn~2+ and SO42- in acid mine wastewater, After seven days of inoculation, the removal rate of TFe, Mn~2+,Zn~2+ and SO42- is 81.96%,35.08%,52.92% and 63.24%,respectively.Additionally, the pH value of the wastewater increases from 4.38 to approximately 6.6.
[1] LUO Y F,WU Y G,FU T L,et al.Effects of a proline solution cover on the geochemical and mineralogical characteristics of high-sulfur coal gangue[J].Acta Geochimica,2018,37(5):701-714.
[2] 张治国，胡友彪，郑永红，等.煤矸石堆存对土壤盐分空间分布特征的影响及主要因子的研究[J].煤炭学报，2018,43(4):1118-1126.ZHANG Z G,HU Y B,ZHENG Y H,et al.Effect of coal gangue stockpiling on spatial distribution characteristics and main factors of soil salinity[J].Journal of China Coal Society,2018,43(4):1118-1126.
[3] 李军，彭传盈，张成业，等.基于大样本的露天开采植被扰动范围一般性统计规律：以神东煤炭基地为例[J].煤炭学报，2023,48(2):975-985.LI J,PENG C Y,ZHANG C Y,et al.General statistical rules of vegetation disturbance range by open-pit mining based on a large sample:a case study of Shendong coal base[J].Journal of China Coal Society,2023,48(2):975-985.
[4] 刘英，魏嘉莉，毕银丽，等.红沙泉露天煤矿碳储量时空动态变化分析[J].煤炭学报，2022,47(增刊1):214-224.LIU Y,WEI J L,BI Y L,et al.Spatiotemporal dynamic change analysis of carbon storage in desertification open-pit mine[J].Journal of China Coal Society,2022,47(Suppl.1):214-224.
[5] 马保国，胡振琪.污泥和粉煤灰覆盖煤矸石山防治污染的模拟试验研究[J].农业环境科学学报，2014,33(8):1553-1559.MA B G,HU Z Q.Simulation experiment on control of coal gangue pollution using sewage-sludge and fly ash covering[J].Journal of Agro-Environment Science,2014,33(8):1553-1559.
[6] 陈胜华，胡振琪，李美生，等.阳泉矿区自燃煤矸石山绿化中覆盖层碾压效果试验[J].水土保持通报，2014,34(1):20-24.CHEN S H,HU Z Q,LI M S,et al.An experiment on rolling compaction effect of covering layer on coal gangue pile with spontaneous combustion in Yangquan city[J].Bulletin of Soil and Water Conservation,2014,34(1):20-24.
[7] 付天岭，吴永贵，姚伦芳，等.水溶性有机质原位覆盖对含硫煤矸石的污染控制[J].环境科学与技术，2014,37(9):42-47.FU T L,WU Y G,YAO L F,et al.In-situ control of pollutants released from coal gangue by use of water-soluble organic materials[J].Environmental Science & Technology,2014,37(9):42-47.
[8] PAGNANELLI F,VIGGI C C,TORO L.Acid mine drainage attenuation by inhibition of pyrite bioleaching using limestone and olive pomace[J].Chemistry and Ecology,2012,28(3):293-303.
[9] JIAO Y N,ZHANG C H,SU P D,et al.A review of acid mine drainage:formation mechanism,treatment technology,typical engineering cases and resource utilization[J].Process Safety and Environmental Protection,2023,170:1240-1260.
[10] 马保国，胡振琪，张明亮，等.高效硫酸盐还原菌的分离鉴定及其特性研究[J].农业环境科学学报，2008(2):608-611.MA B G,HU Z Q,ZHANG M L,et al.Isolation and identification of high efficient sulfate-reducing bacteria and its characteristics[J].Journal of Agro-Environment Science,2008(2):608-611.
[11] 唐婕琳，何环，张文娟，等.SRB菌的分离鉴定及其对矸石山酸性废水的处理[J].煤炭学报，2014,39(11):2307-2314.TANG J L,HE H,ZHANG W J,et al.Isolation and identification of SRB and its utilization on processing of acid mine drainage of coal gangue dump[J].Journal of China Coal Society,2014,39(11):2307-2314.
[12] DONG Y R,DI J Z,YANG Z H,et al.Study on the effectiveness of sulfate-reducing bacteria combined with coal gangue in repairing acid mine drainage containing Fe and Mn[J].Energies,2020,13(4):995.DOI:10.3390/en13040995.
[13] PHAM C T K,SAWADA K,SODA S.Characterization of a moderately Halotolerant antimony-removing Desulfovibrio sp.strain isolated from landfill leachate[J].Water,2023,15(22):3872.DOI:10.3390/W15223872.
[14] YANG Z D,JI N,HUANG J,et al.Decreasing lactate input for cost-effective sulfidogenic metal removal in sulfate-rich effluents:mechanistic insights from (bio)chemical kinetics to microbiome response[J].Chemosphere,2023,330:138662.DOI:10.1016/j.chemosphere.2023.138662.
[15] 刘一帆，萨如拉，金佳瑶，等.不同碳源下芽孢杆菌4B21对环境pH的影响研究[J].内蒙古石油化工，2024,50(8):28-33.LIU Y F,SA R L,JIN J Y,et al.Study on the effects of bacillus 4B21 on pH values around environment under different carbon sources[J].Inner Mongolia Petrochemical Industry,2024,50(8):28-33.
[16] MARSCHNER B,NOBLE D A.Chemical and biological processes leading to the neutralisation of acidity in soil incubated with litter materials[J].Soil Biology and Biochemistry,2000,32(6):805-813.
[17] 张允.硫酸盐还原菌Citrobacter sp.strain YS1对酸性煤矸石的修复实验研究[D].北京：中国矿业大学，2023.ZHANG Y.Experimentalstudy on the remediation of acid coal gangue by sulfate-reducing bacteria Citrobacter sp.strain YS1[D].Beijing:China University of Mining and Technology,2023.
[18] 赵莹.硫酸盐还原菌的分离及金属离子去除机理的研究[D].长春：吉林大学，2016.ZHAO Y.Isolation ofsulfate reducing bacteria from sewage sludge and determination of its heavy-metal-removal mechanism[D].Changchun:Jilin University,2016.
[19] CHAITALI C,MANDAKINI G,INDRANIL M,et al.Minimal medium optimization for soluble sulfate removal by tailor-made sulfate reducing bacterial consortium[J].Bioremediation Journal,2020,24(4):251-264.
[20] LI X H,XIAO H,ZHANG W J,et al.Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel[J].Journal of Oceanology and Limnology,2019,37(2):600-614.
[21] FROLOV E N,KUBLANOV I V,TOSHCHAKOV S V,et al.Thermodesulfobium acidiphilum sp.nov.,a thermoacidophilic,sulfate-reducing,chemoautotrophic bacterium from a thermal site[J].International Journal of Systematic and Evolutionary Microbiology,2017,67(5):1482-1485.
[22] ABDOULAYE T,ALI G Z B,MALICK M,et al.Desulfovibrio senegalensis sp.nov.,a mesophilic sulfate reducer isolated from marine sediment[J].International Journal of Systematic and Evolutionary Microbiology,2017,67(9):3162-3166.
[23] REIS M A M,ALMEIDA J S,LEMOS P C,et al.Effect of hydrogen sulfide on growth of sulfate reducing bacteria[J].Biotechnology and Bioengineering,1992,40(5):593-600.
基本信息:
DOI：10.3969/j.issn.1007-7545.2024.12.019
中图分类号:X75;X172
引用信息:
[1]曹梦竺,王铎,马婷.SRB的分离鉴定及其对酸性矿山废水的处理[J].有色金属(冶炼部分),2024(12):157-166.DOI:10.3969/j.issn.1007-7545.2024.12.019.
基金信息:
国家重点研发计划项目(2019YFC1805003)
2024-09-26
2024
2024-10-25
2024-10-28
2024
1
2024-12-04
2024-12-04